Biotransformation of Cardenolides from Calotropis procera and Their Cytotoxic Potential against Human Mammary Gland Carcinoma Cells

Background: Poekilocerus pictus (Fabricius 1771), a painted grasshopper, sequesters cardenolides from its food plant, the Apple of Sodom or Aak, Calotropis procera (Aiton) W.T. Aiton (Family-Asclepiadaceae). In our present investigation, we were able to isolate Pseudomonas aeruginosa KRK6 intestine responsible for biotransformation cardenolides. Methods: was grown in methanolic extracts and modified detected by Liquid Chromatography-Mass Spectrometry (LCMS) also used induce apoptosis cancer cells (MCF-7 T-47 D). Result: The CPMEP6 found human breast adenocarcinoma (MCF cells-IC50 =6.31±0.4 µg/mL, T-47D = 10.1±1.02 µg/mL). Phosphatidylserine exposure DNA fragmentation suggested treated cells. induced via mitochondrial pathway down-regulating BCL-2 protein expression up-regulating BAX expression.